HISTOCHEMICAL DEMONSTRATION OF AMINOPEPTIDASE

Histochemical demonstration of desialation

Experiments were carried out to assess the susceptibility of normal and inflammatory bowel disease rectal mucus to desulphation and desialation by faecal extracts and by bacterial sialidase. The effects were assessed histochemically using a combined high iron diamine (HID) and alcian blue (AB) stain for sulphomucins and sialomucins. Rectal mucus in biopsies from controls (irritable bowel syndro...

Histochemical Demonstration of Diaphorases and Dehyd .

T HE PROBLEMS involved in the cytochemical demonstration of the activity of oxidative enzyme systems in the cells of bone marrow and peripheral blood relate mainly to methods of fixation and to the ultimate identification of the cells in which the enzyme reaction takes place. Approaches have been suggested for the demonstration of the activity of specific dehydrogenase systems in blood cells, s...

Histochemical demonstration of acetylcholinesterase in neuroblastoma.

The presence of acetylcholinesterase in the tumour cells of neuroblastoma has been shown by enzyme histochemistry. For comparison, some other tumours likely to be found in children and commonly presenting histologically as small cell tumours have also been studied. Acetylcholinesterase activity was seen in rhabdomyosarcoma, but, compared with neuroblastoma, the activity was focal and sparse. On...

Histochemical demonstration of glycogen synthesis in vitro.

Histochemical demonstration of glycogen in neurons of the cat retina.

PURPOSE To demonstrate histochemically the cellular distribution of particulate glycogen in the cat retina and to correlate it with glucose sensitivity of neuronal electrical activities. METHODS Free-floating, ultrathin sections of cat eyes (without glucose challenges) were stained by the periodic acid-thiocarbohydrazide-silver proteinate procedure and examined by electron microscopy. RESUL...